FGF-21 Responsive Luciferase Reporter HEK293 Cell Line

The FGF-21 Responsive Luciferase Reporter HEK293 Cell Line is a HEK293 cell line engineered to express firefly luciferase under the control of the GAL4 upstream activation sequence (UAS). It overexpresses human beta-Klotho (KLB, NM_175737.4) and human Elk-1 (ETS Like-1 protein) transactivation domain (Elk1-TA, amino acids 205-end) fused to the DNA binding domain (DBD) of GAL4 (GAL4 DBD, amino acid 1-147). This allows for quantitative measurement of FGF-21 (Fibroblast growth factor 21) activity through its cognate receptors and downstream signaling networks. This cell line has been validated by stimulation with recombinant human FGF-21 and inhibition by a pan-FGFR inhibitor, Futibatinib. Figure 1: Illustration of the mechanism leading to luciferase expression in response to FGF-21 treatment in FGF-21 Responsive Luciferase Reporter HEK293 Cell Line.Interested in having us perform the screening and profiling of inhibitors or blocking antibodies? Visit our Immunotherapy Cell-Based Screening Services.