TNF alpha (rat) ELISpot Set

Diaclone ELISpot Sets include capture and detection antibodies, Streptavidin - Alkaline Phosphatase conjugated, BSA, BCIP/NTB, blocking reagent, and no, 1, 5, 10, 15, or 20 sterile or non-sterile plates, , Cross reaction: No cross reactivity with other rat cytokines, Incubation time: 3h after cell stimulation, , Additional info: To receive kit plus non-sterile plates (MSIPN), please add 'P' at the end of the Cat N°. To receive kit plus sterile plates (MSIPS), please add 'S' at the end of the Cat N°. Spots read visually or with reader Protein function: Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance. Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6. [The UniProt Consortium]