sVEGFR1/sFLT1 BioAssay(TM) ELISA Kit, Human

sVEGFR1 is also known as sFMS-related tyrosine kinase 1(sFLT1). Oncogene sFLT belongs to the src gene family and is related to oncogene ROS . Like other members of this family, it shows tyrosine protein kinase activity that is important for the control of cell proliferation and differentiation. sFLT is maps to 13q12. sVEGF receptor 1 signaling is essential for osteoclast development and bone marrow formation in colony-stimulating factor 1-deficient mice. The standard product used in this kit is recombinant human sVEGFR1, consisting of 905 amino acids with a molecular mass of 100kD. Intended Use: sVEGFR1/sFLT1 BioAssay(TM) ELISA Kit, Human is a sandwich ELISA quantitative detection of human sVEGFR1/sFLT1 in cell culture supernates, serum and plasma (EDTA). Range: 15.6-10,000pg/ml, Sensitivity: <30pg/ml, Specificity: Natural and recombinant human sVEGFR1/sFLT1, Crossreactivity: No detectable crossreactivity with other relevant proteins, Kit Components: 144047A: Microtiter Strip, 1x96 wells, 144047B: sVEGFR1/sFLT1 Standard, 2x10ng, 144047C: Anti-human sVEGFR1/sFLT1 (Biotin), 130ul, USB Cat No Kit Component 143696: Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (1:100 dilution), 143697: Sample Diluent Buffer, 1x30ml, 143698: Antibody Diluent Buffer, 1x12ml, 143699: ABC Diluent Buffer, 1x12ml, 143700: TMB Color Developing Agent, 1x10ml, 143701: TMB Stop Solution, 2N H2SO4, 1x10ml, Storage and Stability: Store *144047B powder at 4°C. Once reconstituted store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Assay Summary: 1. Add samples and standards and incubate the plate at 37°C for 90 minutes. Do not wash. , 2. Add biotinylated antibodies and incubate the plate at 37°C for 60 minutes. Wash plate 3 times with 0.01M TBS., 3. Add ABC working solution and incubate the plate at 37°C for 30 minutes. Wash plate 5times with 0.01M TBS., 4. Add TMB color developing agent and incubate the plate at 37°C in dark for 25-30 minutes., 5. Add TMB stop solution and read.