Secretin (SCT) BioAssay(TM) ELISA Kit (Mouse)

Secretin (SCT) BioAssay(TM) ELISA Kit (Mouse)
Artikelnummer Größe Datenblatt Manual SDB Lieferzeit Menge Preis
027984.96 96 tests - -

3 - 19 Werktage*

987,00 €
 
Secretin (SCT) BioAssayT ELISA Kit (Mouse) is a competitive inhibition immunoassay for in vitro... mehr
Produktinformationen "Secretin (SCT) BioAssay(TM) ELISA Kit (Mouse)"
Secretin (SCT) BioAssayT ELISA Kit (Mouse) is a competitive inhibition immunoassay for in vitro quantitative measurement of SCT in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Detection Range: 12.35-1000pg/ml, Sensitivity: 4.95pg/ml, Intra-Assay CV: <10%, Inter-Assay CV: <12%, Kit Components: *027984A: Microtiter Plate, 96 wells, Pre-coated, ready to use, *027984B: Standard, 2x1vial , 027984C: Standard Diluent, 1x20ml, *027984D: Detection Reagent A (green), 1x120ul, *027984E: Detection Reagent B (red), 1x120ul , 027984F: Assay Diluent A, 1x12ml, 027984G: Assay Diluent B, 1x12ml, 027984H: TMB Substrate, 1x9ml, 027984K: Stop Solution, 1x6ml, 027984L: Wash Buffer, 30X, 1x20ml, Precaution: The Stop Solution (027984K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material. Storage and Stability: Store *027984A, *027984B, *027984D and *027984E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Assay Procedure Summary: 1. Prepare all reagents, samples and standards,, 2. Add 50ul standard or sample to each well., 3. Add 50ul prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C., 4. Aspirate and wash 3 times., 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C., 6. Aspirate and wash 5 times., 7. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37°C., 8. Add 50ul Stop Solution. Read at 450nm immediately.
Schlagworte: Sct, Secretin
Hersteller: United States Biological
Hersteller-Nr: 027984

Eigenschaften

Anwendung: ELISA
Spezies-Reaktivität: mouse
Format: Solid Phase

Handhabung & Sicherheit

Lagerung: vT
Versand: +4°C (International: +4°C)
Achtung
Nur für Forschungszwecke und Laboruntersuchungen: Nicht für die Anwendung im oder am Menschen!
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