Rat alpha2 macroglobulin, alpha2 MG ELISA Kit

Sample Types: serum, plasma Detection Range: 3.13 ng/mL-800 ng/mL Sensitivity: 3.125 ng/mL Assay Principle: quantitative Measurement: CompetitiveAssay Time: 1-5h Sample Volume: 50-100ulDetection Wavelength: 450 nmProtein function: Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase. [The UniProt Consortium]