Progranulin (human) BioAssay(TM) ELISA Kit (Proepithelin, PEPI, PC Cell-derived Growth Factor)

Progranulin (PGRN) is a widely expressed pluripotent growth factor which plays a role in processes such as development, wound repair and inflammation by activating signaling cascades that control cell cycle progression and cell motility. Its function in the central nervous system is of interest, as mutations in the PGRN gene were found in cases of frontotemporal degeneration (FTLD). In addition, PGRN has also been linked to tumorigenesis. New cutting-edge publications have shown that progranulin might be a biomarker not only for FTLD, but also for other types of Alzheimer's disease (AD). This new data even supposes the potential of progranulin to be a biomarker for MCI (Mild Cognitive Impairment), which would be a hallmark in AD research. Additionally, PGRN is described as a new ligand of TNF receptors and a potential therapeutic against inflammatory disease like arthritis. Sample Type: Cell Culture Supernatant, Plasma, Serum, Urine , Intended Use: The Progranulin (human) ELISA Kit is to be used for the in vitro quantitative determination of human progranulin in serum, plasma, urine, cell culture supernatant and cerebrospinal fluid (CSF, R. Ghidoni et al., Neurology 71, 1235 (2008)). Sensitivity: 32pg/ml, Range: 0.063-4ng/ml , Specificity: Detects human progranulin. Test Principle: This assay is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for quantitative determination of human progranulin in biological fluids. A polyclonal antibody specific for progranulin has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, progranulin is recognized by the addition of a biotinylated polyclonal antibody specific for progranulin (Detection Antibody). After removal of excess biotinylated antibody, HRP labeled streptavidin (STREP-HRP) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3',5,5'-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450 nm after acidification and is directly proportional to the concentration of progranulin in the samples. Kit Components: Coated plate with human progranulin Antibody , 1x1, Wash Buffer 10X, 2x30ml, ELISA Buffer 10X, 2x30ml, Detection Antibody, 1x30ul, HRP Labeled Streptavidin (lyophilized), 1x2ug, human progranulin Standard (lyophilized), 1x8ng , TMB Substrate Solution, 1x12ml, Stop Solution, 1x12ml, 2 plate sealers (plastic film) , 2 silica Gel Minibags, Storage and Stability: Store *xxx powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.