Mouse NEP(Neprilysin) ELISA Kit

Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids (By similarity). Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond (By similarity). Catalyzes cleavage of bradykinin, substance P and neurotensin peptides (By similarity). Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9 (By similarity). Involved in the degradation of the atrial natriuretic factor (ANF) (By similarity). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers (PubMed:20876573) Assay Type: Sandwich. Sensitivity: 21.3 pg/mL. Standard: 5000 pg/mL. Detection range: 78.13-5000 pg/mL. Sample type: Serum, plasma and other biological fluids.. Assay length: 3.5h. Research Field: Enzyme & Kinase,Neuro science,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse NEP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NEP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse NEP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NEP in the samples is then determined by comparing the OD of the samples to the standard curve.