Mouse FKBP1B (FK506 Binding Protein 1B) Microsample ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse FKBP1B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse FKBP1B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse FKBP1B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse FKBP1B in the samples is then determined by comparing the OD of the samples to the standard curve.Detection Range: 0.79-50 ng/mL. Sensitivity: 0.34 ng/mL. Assay Type: Sandwich. Standard: 50 ng/mL. Sample Type: sTissue homogenates, cell lysates and other biological fluids.. Assay duration: 3.5h. Protein Function: Has the potential to contribute to the immunosuppressive and toxic effects of FK506 and rapamycin. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides (By similarity) [The Uniprot Consortium]