Interleukin-1 beta, Human, BioAssay(TM) ELISA Kit (IL-1 beta, Catabolin, IL-1B, IL1F2, IL1-beta)

Sandwich ELISA quantitative detection kit for Human IL-1 beta in cell culture supernates, serum and plasma (heparin, EDTA, citrate). Interleukin-1b ( IL-1b) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1b, a prominent microglia-derived cytokine, caused oligodendrocyte death in coculture with astrocytes and microglia, but not in pure culture of oligodendrocytes alone. It also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B (NFKB)-regulated genes2. Furthermore, Microenvironmental IL-1b and, to a lesser extent, IL-1a are required for in vivo angiogenesis and invasiveness of different tumor cells. Additional, the cooperation of IL-1b and PDGFB induces contractile-to-synthetic phenotype modulation of human aortic smooth muscle cells in culture. Moreover, the association with disease may be explained by the biologic properties of IL-1b, which is an important proinflammatory cytokine and a powerful inhibitor of gastric acid secretion. , Assay Range:, 3.9-250pg/ml, Sensitivity: <0.15 pg/ml, Specificity: Recognizes native and recombinant human IL-1b. Crossreactivity: There is no detectable cross-reactivity with other relevant proteins. Uniprot ID: P01584, Entrez Gene ID: 3553, Kit Components: 143885A: Microtiter Strip, 1x96-well Pre-coated with anti-IL-1 beta, Human , 143885B: Standard, 1000pg/ml, 2x1vial Lyophilized recombinant IL-1 beta, Human , 143885C: Pab (Biotin) GtxHu, 1x130ul, USB Cat No Kit Component, 143696: Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (1:100 dilution), 143697: Sample diluent buffer, 1x30ml, 143698: Antibody diluent buffer, 1x12ml, 143699: ABC diluent buffer, 1x12ml, 143700: TMB color developing agent, 1x10ml, 143701: TMB stop solution, 2N H2SO4, 1x10ml, Storage and Stability: Store *143885B powder at 4°C. Once reconstituted store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Summary 1. Add samples and standards and incubate the plate at 37°C for 90 minutes. Do not wash. , 2. Add biotinylated antibodies and incubate the plate at 37°C for 60 minutes. Wash plate 3 times with 0.01M TBS. , 3. Add ABC working solution and incubate the plate at 37°C for 30 minutes. Wash plate 5 times with 0.01M TBS. , 4. Add TMB color developing agent and incubate thutese plate at 37°C in dark for 25-30 min. , 5. Add TMB stop solution and read.