Furin BioAssay(TM) ELISA Kit, Human

Sandwich ELISA quantitative detection kit for Human Furin in cell culture supernates, tissue homogenates and cell lysates. Furin(Fur), also known as PACE, is a protein that in humans is encoded by the FURIN gene. The protein encoded by this gene is an enzyme which belongs to the subtilisin-like proprotein convertase family. The FUR gene is located approximately 1 kb upstream of the FES gene on chromosome 15q25-q26. This gene is thought to play a role in tumor progression. Furin is one of the proteases responsible for the proteolytic cleavage of HIV envelope polyprotein precursor gp160 to gp120 and gp41 prior to viral assembly. Furin is enriched in the Golgi apparatus, where it functions to cleave other proteins into their mature/active forms. Expression of furin in T-cells is required for maintenance of peripheral immune tolerance , Assay Range:, 312pg/ml-20,000pg/ml, Sensitivity: < 10 pg/ml , Crossreactivity: There is no detectable cross-reactivity with other relevant proteins. Uniprot ID: P09958, Entrez Gene ID: 5045, Kit Components: 96-well plate pre-coated with anti-Furin, Human, Lyophilized recombinant Furin, Human standard, 10ng/tube, 2Ea, USB Cat No Kit Component, 143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution), 143697 Sample diluent buffer, 30ml, 143698 Antibody diluent buffer, 12ml, 143699 ABC diluent buffer, 12ml, 143700 TMB color developing agent, 10ml, 143701 TMB stop solution, 10ml, Storage and Handling: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.