EasyStep Human FE (Ferritin) ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FE, and the Human FE standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human FE. After TMB substrate solution is added, only those wells that contain Human FE and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FE in the samples is then determined by comparing the OD of the samples to the standard curve. Detection Range: 0. 32-20 ng/mL. Sensitivity: 3. 2 pg/mL. Assay Type: Sandwich. Standard: 20 ng/mL. Sample Type: Serum. Assay duration: 1. 5h