SUV4-20H1 Chemiluminescent Assay kit

The SUV4-20H1 Chemiluminescent Assay Kit is designed to measure SUV4-20H1 activity for screening and profiling applications. The SUV4-20H1 Chemiluminescent Assay Kit comes in a convenient format, with 96-well plate precoated with histone H4 substrate, an antibody against methylated lysine residue of Histone H4, HRP-labeled secondary antibody, S-adenosylmethionine, methyltransferase assay buffer, and purified SUV4-20H1 enzyme for 96 enzyme reactions. The key to the SUV4-20H1 Chemiluminescent Assay Kit is a highly specific antibody that recognizes a methylated residue of Histone H4. With this kit, only three simple steps are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.