SetD2 Chemiluminescent Assay Kit

The SETD2 Chemiluminescent Assay Kit is designed to measure SETD2 activity for screening and profiling applications. The SETD2 Chemiluminescent Assay Kit comes in a convenient strip format, with a 96-well plate precoated with histone H3 peptide substrate, the antibody against methylated lysine36 residue of Histone H3, the secondary HRP-labeled antibody, S-adenosylmethionine, methyltransferase assay buffer, and purified SETD2 enzyme for 100 enzyme reactions. The key to the SETD2 Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated K36 residue of Histone H3. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.