PRMT5 chemiluminescent assay kit

The PRMT5 Direct Activity Assay kit is designed to measure PRMT5 activity using purified PRMT5 or PRMT5 extracts. The PRMT5 Direct Activity Assay Kit comes in a convenient format, with a 96-well plate precoated with histone H4 peptide substrate, the antibody against methylated arginine residue of Histone H4, the secondary Europium-labeled antibody, S-adenosylmethionine, methyltransferase assay buffer, and purified PRMT5 enzyme for 100 enzyme reactions. The key to the PRMT5 Direct Activity Assay Kit is a highly specific antibody that recognizes methylated R3 residue of Histone H4. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the plates are treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.