PRMT4 chemiluminescent assay kit

The PRMT4 direct activity assay kit is designed to measure PRMT4/CARM1 activity using purified PRMT4 or cell extracts containing PRMT4. The PRMT4 direct activity assay kit comes in a convenient format, with a 96-well plate precoated with the specific PRMT4 peptide substrate, the antibody against methylated substrate, the secondary HRP-labeled antibody, S-adenosylmethionine, methyltransferase assay buffer, and purified PRMT4 enzyme for 100 enzyme reactions. The key to the PRMT4 Direct Activity Assay Kit is a highly specific antibody that recognizes methylated PRMT4 substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.