PDE5A Assay Kit

The PDE5A Assay Kit is designed for identification of inhibitors of PDE5A using fluorescence polarization. Phosphodiesterases (PDEs) play an important role in dynamic regulation of cAMP and cGMP signaling. PDE5A, also known as cGMP-inhibited phosphodiesterase, has been implicated in cardiovascular function and fertility. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by PDE5A to the binding agent. The PDE5A inhibitor screening assay kit comes in a convenient 96-well format, with purified PDE5A enzyme, fluorescently labeled PDE5A substrate (cGMP), binding agent, and PDE assay buffer for 100 enzyme reactions. The key to the PDE5A Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for PDE5A reactions. First, the fluorescently labeled cGMP is incubated with a sample containing PDE5A for 1 hour. Second, a binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader.