NRG1beta: HER4 (ERBB4) Chemiluminescent Assay Kit

The NRG1beta: HER4 (ERBB4) Chemiluminescent Assay Kit is an ELISA-based assay designed to measure the binding between NRG1beta (neuregulin-1beta) and HER4 (human epidermal growth factor receptor 4, also known as ERBB4) for screening and profiling applications. The NRG1beta: HER4 (ERBB4) Chemiluminescent Assay Kit comes with enough purified NRG1beta (amino acids 2-246) and HER4 (amino acids 26-651) proteins, detection antibody, assay buffer, and detection reagent for 100 enzyme reactions. A 96-well plate is coated with NRG1beta protein. After coating and blocking, HER4 is added in an optimized assay buffer. Unbound HER4 is washed away, and the plate is incubated with a detection antibody. Finally, ELISA ECL substrate is added to produce chemiluminescence that can be measured using a chemiluminescence reader. The chemiluminescence signal is proportional to the efficacy of HER4 binding to NRG1beta.