Na+k+-ATPase Activity Assay Kit

Detection principle Na+K+-ATPase decomposes ATP to produce ADP and Phosphorus, and calculates the activity of Na+K+-ATPase by measuring the content of phosphorus. Performance characteristics Synonyms: Na+K+-ATPase Sample type: Serum, plasma, animal tissue Sensitivity: 0.11 µmol Pi/mL/hour Detection range: 0.42-4.99 µmol Pi/mL Detection method: Colorimetric method Assay type: Enzyme Activity Assay time: 35 min Precision: Average inter-assay CV: 2.2%Average intra-assay CV: 2.2% Other instruments required: Micropipettor, Tubes, Vortex mixer, Incubator, 37? Water bath, Centrifuge. Other reagents required: Normal saline (0.9% NaCl) Storage: 2-8? Valid period: 6 months Dilution of sample It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range ( 0.42-4.99 µmol Pi/mL/hour). The recommended dilution factor for different samples is as follows (for reference only): Sample type Dilution factor Rat plasma 1 10% Rat liver tissue homogenate 6-10 10% Rat spleen tissue homogenate 4-8 10% Rat heart tissue homogenate 4-8 10% Mouse brain tissue homogenate 4-8 Note: The diluent is normal saline (0.9% NaCl).