Mitochondrial Complex II Activity Assay Kit

Detection instrument: Microplate reader. Detection principle: Coenzyme Q, the catalytic product of mitochondrial complex II, can further reduce 2, 6-dichloroindoxol, which has a characteristic absorption peak at 600 nm. Therefore, the activity of mitochondrial complex II can be quantified by measure the change OD value at 600 nm. Sample type: animal tissue, cell. Research area: Enzymes,Tricarboxylic Acid(TCA) Cycle. Assay time: 15 min. Sensitivity: 0.54 U/L. Detection range: 0.54-17.66 U/L. Recovery rate: 97. Inter CV: 5. Intra CV: 4