JMJD2C Assay Kit

The JMJD2C Direct Activity Assay Kit is designed to measure activity of the JMJD2C using purified enzyme complex or cell extracts containing JMJD2C. JMJD3 is a JmjC-domain protein that exhibits demethylation activity toward H3-K9Me3 and H3-K36Me3. The JMJD2C Assay Kit comes in a convenient format, with a 96-well plate, precoated with the methylated histone H3 peptide substrate, primary antibody, the secondary HRP-labeled antibody, demethylase assay buffer, and purified JMJD2C for 100 enzyme reactions. The key to the JMJD2C Direct Activity Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing JMJD2C enzyme is incubated with a sample containing assay buffer for two hours. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.