Glutaminase (GLS) Activity Assay Kit

Detection instrument: Microplate reader. Detection principle: Glutamine is decomposed to produce glutamic acid under the action of glutaminase. Glutamic acid is further transformed by glutamic acid dehydrogenase. Meanwhile, NAD+ is reduced to NADH, Which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The activity of glutaminase can be calculated by measuring the change of absorbance value at 450 nm. Sample type: serum (plasma), animal, plant tissue. Research area: Enzymes,Amino acids and proteins. Assay time: 80min. Sensitivity: 0.003 U/L. Detection range: 0.003-18.0 U/L. Inter CV: 8.3. Intra CV: 5