G9a chemiluminescent assay kit

The G9a Direct Activity Assay kit is designed to measure G9a activity using purified G9a or G9a extracts. The G9a Direct Activity Assay Kit comes in a convenient format, with a 96-well plate precoated with histone H3 peptide substrate, the antibody against methylated lysine residue of Histone H3, the secondary Europium-labeled antibody, S-adenosylmethionine, methyltransferase assay buffer, and purified G9a enzymes for 100 enzyme reactions. The key to the G9a Direct Activity Assay Kit is a highly specific antibody that recognizes methylated K9 residue of Histone H3. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the plates are treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.