EHMT1/GLP Chemiluminescent Assay Kit

The EHMT1 (GLP) Chemiluminescent Assay Kit is designed to measure EHMT1 (also known as G9a-like protein) activity for screening and profiling applications. The EHMT1 (GLP) Chemiluminescent Assay Kit comes in a convenient format, with a 96-well plate precoated with histone H3 peptide substrate, primary antibody against methylated lysine residue of Histone H3, the secondary HRP-labeled antibody, S-adenosylmethionine, methyltransferase assay buffer, and purified EHMT1 for 100 enzyme reactions. The key to the EHMT1 (GLP) Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated K9 residue of H3. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primaryantibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader. Note: The kit is also suitable for use with cell extracts, but non-specific methyltransferase activity may be detected.