BRD4 (BD2) Inhibitor Screening Assay Kit

The BRD4 (BD2) Inhibitor Screening Assay Kit is designed to measure the inhibition of BRD4 bromodomain 2 (BD2) binding to its substrate. The BRD4 (BD2) Inhibitor Screening Assay Kit comes in a convenient AlphaLISA® format, with biotinylated histone peptide substrate, assay buffer, detection buffer and purified BRD4 BD2 to perform a total of 384 enzyme reactions. The key to the BRD4 Inhibitor Screening Assay Kit is the highly specific binding of the BRD4 bromodomain to the acetylated histone substrate. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing BRD4 and an inhibitor of choice is incubated with the biotinylated substrate for thirty minutes. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts.