BRD4 (BD1 and BD2) TR-FRET Assay Kit

The BRD4 (BD1 + BD2) TR-FRET Assay Kit is designed to measure the inhibition of BRD4 (BD1 + BD2) binding to its substrate in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple, a sample containing terbium-labeled donor, dye-labeled acceptor, BRD4, substrate, and an inhibitor is incubated for sixty minutes. Then, the fluorescence intensity is measured using a fluorescence reader.