ATAD2B Inhibitor Screening Assay Kit

The ATAD2B Inhibitor Screening Assay Kit is designed to measure the inhibition of ATAD2B binding to its substrate. The kit comes in a convenient AlphaLISA format, with enough biotinylated histone peptide substrate, assay buffer, detection buffer and purified GST-tagged ATAD2B bromodomain to perform a total of 384 enzyme reactions. The key to the kit is the specific binding of the ATAD2B bromodomain to the acetylated histone substrate. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing ATAD2B and an inhibitor of choice is incubated with the biotinylated substrate for thirty minutes. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts.