Amplite(TM) Fluorimetric Pyruvate Assay Kit

Ex: 571 nm. Em: 585 nm. Pyruvate is an important chemical compound in intracellular metabolic pathways. It is derived from metabolism of glucose known as glycolysis. One molecule of glucose breaks down into two molecules of pyruvate, which supplies living cells energy through one of two ways. When oxygen is present (aerobic respiration), pyruvate is converted into acetyl-CoA by pyruvate dehydrogenase which enters citric acid cycles (also known as the Krebs cycle) to generate ATP. When there is insufficient oxygen is available, the pyruvate is broken down anaerobically, creating lactate in animals and ethanol in plants and microorganisms. Abnormal levels of pyruvate, or concentration ratio of lactate-to-pyruvate may be linked to liver disease or metabolic disorders and it is a diagnostic measurement in patient's clinical and other laboratory studies. AAT Bioquest's AmpliteT Colorimetric Pyruvate Assay Kit offers a sensitive colorimetric assay for quantifying pyruvate in the samples. It utilizes an enzyme coupled reaction that releases hydrogen peroxide, which can be detected by pyruvate sensor by an absorbance microplate reader at 575 nm.