Yeast Nitrogen Base

Yeast Nitrogen Base is nutrient-rich medium with sources of nitrogen, vitamins, trace elements and salts. It acts as the basic nutrient medium for most fermentation reactions involving yeast cultures. Yeast Nitrogen Base with and without added amino acids for the cultivation of yeast and for classification based on amino acid and carbon requirements, respectively. Yeast Nitrogen Base is based upon the formulation of Yeast Agar except that Ammonium Sulfate is the sole nitrogen source and that addition of carbon source is required. The medium may be used to test carbon assimilation in the presence of a carbon source like glucose, galactose or raffinose Suspend 6.9 gram powdered medium in 1 litre distilled water.