SIH Medium

Formula (mg/l): Amino Acids  , Arg 700 Asp 300 Asp A 150 Cys 300 GluA 545 Gly 900 His 300 Ile 600 Leu 900 Lys 1250 Met 350 Phe 550 Pro 800 Thr 500 Trp 350 Val 700 Vitamins  , Biotin 0.02 Cyanocobalamin 0.01 Folic Acid 0.2 Lipoic Acid 0.4 Riboflavin 0.5 Thiamine 0.6 Micro Elements  , Na2EDTA.2H2O 4.84 ZnSO4 2.3 H3BO3 1.11 MnCl2.4H2O 0.51 CoCl2.6H2O 0.17 CuSO4.5H2O 0.15 (NH4)6Mo7O24.4H2O 0.1 Minerals  , NH4Cl 53.5 CaCl2.2H2O 2.94 FeCl3 16.2 MgCl2.6H2O 81.32 KH2PO4 870 Carbon Source  , Glucose 10000 Total 20326 Suspend 20.3 gram powdered medium in 1 litre distilled water. Store dry at room temperature.