FM Minimal Medium

Formula (mg/l): Amino Acids  , Arg 700 Asp 300 Cys 200 GluA 500 Gly 900 His 300 Ile 600 Leu 900 Lys 900 Met 300 Phe 500 Pro 800 Thr 500 Trp 200 Val 700 Vitamins  , Biotin 0.02 Cyanocobalamin 0.01 Folic Acid 0.2 Lipoic Acid 0.4 Riboflavin 0.5 Thiamine 0.6 Micro Elements  , Na2EDTA.2H2O 4.84 ZnSO4 2.3 H3BO3 1.11 MnCl2.4H2O 0.51 CoCl2.6H2O 0.17 CuSO4.5H2O 0.15 (NH4)6Mo7O24.4H2O 0.1 Minerals  , NH4Cl 53.5 CaCl2.2H2O 2.94 FeCl3 16.2 MgCl2.6H2O 81.32 KH2PO4 870 Carbon Source  , Glucose 10000 Total 19334.87 Suspend 19.3 gram powdered medium in 1 litre distilled water. Store dry at room temperature.