Endothelial Cell Growth Supplement (ECGS), highly purified

ECGS is mitogenic for many types of cells (i.e. mammalian, avian and human endothelial cells, smooth muscle cells, keratinocytes, melanocytes and hybridomas) under reduced or serum-free conditions. It often fully substitutes for feeder layers in culture of fastidious cells. Bovine hypothalamic protein extract, is rich in cell growth and attachment factors. Range for Optimal Activity: Use in culture at 10-100ug/ml, Biological Activity: 15ug/ml supports 4.6-fold growth of fetal bovine heart endothelial cells cultured for 5 days in 50% RPMI/50% DMEM (RD) medium, 2% FBS. Results may vary depending on cell line used. Storage and Stability: Lyophilized powder is stable for 12 months at -20°C. Reconstituted product is stable for 3 months at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.