CHO Serum-Free Basal Medium

Applicable cell lines: CHO serum-free basal medium is a fully chemically defined (CD) basal medium that does not contain hydrolysates, proteins, growth factors, or any animal-derived components. It is suitable for high-density suspension culture of different subtypes of Chinese hamster ovary cells (CHO-K1, CHODG44, CHOZN, etc.). With high-performance feed (CHO serum-free feed A, CHO serum-free feed B) medium, it can support high-density cell growth and viability maintenance, and achieve higher levels of protein/antibody expression and quality. Medium characteristics: 1. Chemically-Defined, CD. 2. Free of hydrolysates, proteins, growth factors or ingredients of any animal origin. 3. Free of HT and L-glutamine. Culture conditions 1. The culture temperature is 37 °C, and the recommended initial cell seeding density is 0.8-1.2×10^6cells/mLin batch and fed-batch culture process. 2. Under the condition of non-automatic pH control, it is recommended to control the CO2 concentrationat 5%. 3. Process conditions such as pH, DO and temperature can be set according to process development results or platform process parameters.