GenePORTER 2 DNA Diluent

PROTOCOL FOR DNA DILUENT DIRECTIONS * Hydrate GenePORTER 2 lipid film at room temperature with hydration buffer. Vortex for 10 seconds at top speed before use. Store the hydrated reagent at 4o C and vortex briefly before each use. * Use the DNA diluent to prepare the DNA solution. Use 25 µl of diluent for 1 µg DNA. Avoid vortexing the DNA diluent solution. * For most cell types, use 5 µl of GenePORTER 2 reagent with 1 µg of DNA.