BioPORTER Protein Delivery Reagent QuikEase Kit

BioPORTER Protein Delivery Reagent QuikEase Kit. The BioPORTER? Protein Delivery Reagent is an efficient and trusted reagent for intracellular delivery of bioactive molecules, such as proteins, peptides, and antibodies, into a broad range of cell types. Although there are many effective reagents available to introduce transcriptionally active DNA into viable cells, The BioPORTER Reagent was designed specifically for the delivery of functional peptides and proteins into living cells, using a unique lipid-based carrier system. The BioPORTER Reagent is effective, easy to use, and more economical than both microinjection and electroporation for the delivery of biologically active proteins into living cells. The specific formulation of the BioPORTER Reagent can deliver various molecules, over a broad range of cell types, in serum-free conditions, and within 3 to 4 hours of incubation. Various molecules, such as fluorescent-antibody, high and low molecular weight dextran sulfate, phycoerythrin-BSA, beta-galactosidase, caspase 3, caspase 8, and granzyme B have been successfully delivered with the BioPORTER Reagent into the cytoplasm of a variety of different adherent and suspension cells+. Furthermore, apoptotic proteins such as granzyme B, caspase 3, or caspase 8 drove cells into apoptosis after delivery with the BioPORTER Reagent, confirming that BioPORTER delivers functional proteins into cells. The QuikEase(TM) single-use tube kit format make it more convenient to use the BioPORTER Reagent. Each tube allows for one reaction in a 6-well dish. The BioPORTER QuikEase kits make it easier than ever to deliver your macromolecules directly for intercellular signaling, cell cycle regulation, control of apoptosis, study of oncogenesis, and transcription regulation studies to name a few. The dried BioPORTER Reagent formulation is first dissolved in a solvent and aliquoted into small eppendorf tubes according to the type of assays conducted (see Methods and Procedures). After complete drying, the BioPORTER Reagent is formulated with a solution of the protein or peptide to be delivered. The BioPORTER Reagent reacts quickly and interacts non-covalently with the protein, peptide, or other molecule, creating a protective vehicle for immediate delivery into cells. The hydrated mixture is then added onto cells, and the BioPORTER/protein complexes attach to negatively charged cell surfaces. The BioPORTER Reagent can then fuse directly with the plasma membrane and deliver the captured protein into the cells (see "1" in the figure to the right), or the BioPORTER-protein complexes are endocytosed by the cells and then fuse with the endosome, releasing the BioPORTER-captured protein into the cytoplasm (see "2" in the figure to the right). Delivery of molecules with the BioPORTER Reagent is very easy and requires only 4 hours of incubation with the target cells.