One-step NZYSpeedy qPCR Green kit

One-step NZYSpeedy RT-qPCR Green kit is an optimized and highly efficient reaction mixture developed for first-strand cDNA synthesis and subsequent real-time PCR in a single tube. The kit includes a One-step NZYSpeedy qPCR Green master mix developed for fast PCR, provided as a 2x reaction mixture, which contains all components necessary for real-time PCR, including a green intercalating dye, dNTPs, stabilizers and enhancers. In addition, a separate NZYRT mix that comprises a balanced mixture of both Reverse transcriptase and Ribonuclease Inhibitor is also provided. This kit enables fast and highly reproducible procedures on the most common real-time PCR apparatus from either total RNA or mRNA. It is optimized for intercalating green dye detection on different instruments. The latest developments in PCR enhancers have been incorporated in the One-step NZYSpeedy RTqPCR Green kit, including buffer chemistry and a polymerase with hot-start-like activity. This optimized mixture together with the NZYRT mix results in highly sensitive and successful results. Components: * One-step NZYSpeedy qPCR Green master mix (2x) * NZYRT mix * DEPC-treated H2O. Shipping Conditions: Dry Ice. Storage Conditions: -85°C to -15°C. Starting Material: RNA. Features: Fast, Hot-start. Detection Method: Intercalating dye. Reference Dye: No ROX. Format: Kit, Liquid. Product Category: Real-Time PCR. Compatibility: Analytik Jena qTOWER3, Analytik Jena Toptical, Bio-Rad CFX connect, Bio-Rad CFX Opus, Bio-Rad CFX96 Touch, Bio-Rad CFX96 Touch Deep Well, Bio-Rad Chromo4(TM), Bio-Rad iCycler(TM) IQ, Bio-Rad iCycler(TM) IQ 4, Bio-Rad iCycler(TM) IQ 5, Bio-Rad iCycler(TM) MiniOpticon, Bio-Rad MyiQ(TM), Bio-Rad Opticon2(TM), Bio-Rad Opticon(TM), Bioer Gene Touch 96, Illumina Eco(TM), Roche LightCycler(R) 480, Roche LightCycler(R) 96, Roche LightCycler(R) Nano, Rotor-Gene(TM) 3000, Rotor-Gene(TM) 6000, Rotor-Gene(TM) Q. Protocol Time: Approx. 60 min. Hot Start: Yes