NZYSupreme One-step RT-qPCR Probe Master Mix (2x), ROX

NZYSupreme One-step RT-qPCR Probe Master Mix, ROX is an optimized and highly efficient reaction mixture developed for first-strand cDNA synthesis and subsequent real-time PCR in a single tube. This offers great convenience and minimizes the risk of errors and contaminations. The master mix, formulated as a 2x reaction mixture, contains all components necessary for both cDNA synthesis and real-time PCR (including enzymes, dNTPs, stabilizers and enhancers), except primers, probes and RNA template. This master mix was engineered with a dual hot-start enzyme control mechanism to provide the highest detection sensitivity. In addition, the latest developments in PCR enhancers have been incorporated in the NZYSupreme One-step RT-qPCR Probe Master Mix, including buffer chemistry and incorporation of highly robust engineered enzymes. Components: NZYSupreme One-step RT-qPCR Probe Master Mix (2x), ROX. Starting Material: RNA. Features: Dual Hot-start, Multiplex, Ultra-sensitive. Detection Method: Probe. Reference Dye: ROX. Format: Liquid, Mastermix. Product Category: Real-Time PCR. Compatibility: AB(TM) 7500, AB(TM) 7500 Fast, AB(TM) QuantStudio(TM) 12K, AB(TM) QuantStudio(TM) 3, AB(TM) QuantStudio(TM) 5, AB(TM) QuantStudio(TM) 6, AB(TM) QuantStudio(TM) 7, AB(TM) QuantStudio(TM) ViiA7(TM), Agilent AriaMx, Agilent Mx3000P(TM), Agilent Mx3005P(TM), Agilent Mx4000(TM), Fluidigm(R) Biomark(TM). Concentration: 2x. Protocol Time: Approx. 60 min. Hot Start: Yes