Multiplex PCR NZYTaq 2x Colourless Master Mix

Multiplex PCR NZYTaq 2x Colourless Master Mix is a premixed ready-to-use solution designed for the simultaneous amplification of multiple DNA fragments (up to 15 targets) in a single tube. The Master Mix contains dNTPs and reaction buffer at optimal concentrations for efficient multiplexing of targets ranging in size from 70 bp to 2.5 kb over a broad range of primer and template concentrations. MgCl2 final concentration is 2.5 mM, allowing the implementation of a comprehensive variety of PCR protocols. Amplification efficiency is further improved by the inclusion of a recombinant modified enzyme derived from Taq DNA polymerase, which was engineered to display a hot-start-like PCR capacity and high processivity (fast polymerisation). The enzyme is inactive at room temperature, avoiding extension of non-specifically annealed primers or primer-dimers, and thus ensuring highly specific and sensitive multiplex PCR amplification. Extension time: 30 sec/kb at 72 °C 3'->5' activity: no Product overhang: 3'-A. Components: Multiplex PCR NZYTaq 2x Colourless Master Mix (0.2 U/µL). Shipping Conditions: Blue Ice. Storage Conditions: -85°C to -15°C. Application: Multiplex PCR, TA Cloning, Typing/Detection. Features: Hot-start, Multiplex. Product Length: 0-3 kb. Format: Mastermix. Product Category: End-Point PCR. Concentration: 0.2 U/µL. Hot Start: Yes