BlockOut(R) Universal Blocking Buffer for Western Blotting 3-PACK (3x500mL)

Blocking buffer was prepared using ultra pure reagents dissolved in pharmaceutical grade water (WFI) and consists of a proprietary protein formulation in TRIS buffered saline at pH 7.6 with thimerisol added as an antimicrobial agent. BlockOut(R) Universal Blocking Buffer Solution for Western Blotting is specifically designed for colorimetric, chemiluminescent, and fluorescent western blotting. BlockOut(R) is recommended for blocking when phospho specific antibodies are used. Pure nitrocellulose membrane is recommended for maximum performance. Other membranes, such as PVDF or nitrocellulose embedded in a support can be used, but may generate elevated backgrounds. Protein should be transferred from gel to membrane using standard protocols. BlockOut(R) Blocking buffer can be used for membrane blocking and to dilute both primary and secondary antibodies. BlockOut(R) is suitable for use with chemiluminiscent and fluorescent western blot imaging systems produced by Bio-Rad Laboratories, GE Healthcare, Alpha Innotech, FujiFilm Life Science, Licor Biosciences, UVP and Syngene.