2x General Serum Diluent, SD1, 1L

2X General Serum Diluent is formulated for diluting goat, rabbit, mouse, avian, and human sera, as well as mouse ascites fluid and cell culture media, for testing in sandwich ELISA configurations. It can also be used for dilution of many types of serum samples in antigen-down ELISA formats. Our 2X concentrated version is helpful for shipping purposes and produces less waste. General Serum Diluent is formulated to provide a stable, protein-friendly environment for dilution of serum, cell culture, ascites, urine, and other aqueous-based biological samples for evaluation within an ELISA format. It can serve as an excellent generic matrix in which to dilute antigen standards when preparing standard curves within antibody-sandwich formats. It also provides an excellent serum dilution medium for antigen down ELISAs in the assessment of a humoral immune response to a particular disease state or immunization regimen. General Serum Diluent provides a mammalian protein-buffered, neutral pH environment that is highly compatible with antibody-antigen interactions. Inclusion of a protein component helps minimize the degree of non-specific IgG adsorption onto coated and blocked ELISA plate wells during antigen-down screening of serum or plasma samples. Bulk volumes and custom packaging are available upon request. Protocol: 1. Prepare 1X working strength sample diluent by diluting 1:2 in diH2O. Add 1 part General Serum Diluent, 2X to 1 part diH2O. Mix well. 2. Prepare standards and samples in General Serum Diluent. 3. Generally, serum samples should be diluted at least 1:50 in order to minimize backgrounds caused by non-specific antibody binding. Highly concentrated samples may need to be diluted 1:1,000 or more. 4. To dilute the sample 1:100, add 1 part sample to 99 parts General Serum Diluent. For example, add 10 µL sample to 990 µL General Serum Diluent for a total of 1,000 µL. 5. Once diluted, run the assay according to the specific ELISA protocol. 6. Analyze the data. If the samples were diluted, the dilution factors must be considered when calculating their values.