10× RBC Lysis/Fixation Solution

10xRBC Lysis/Fixation Solution is designed for FCM sample processing of human whole blood. The whole blood is treated with anticoagulant and incubated with fluorescent antibody, the whole blood is added with the RBC Lysis/Fixation Solution to lyse excess red blood cells for FCM. When added to the whole blood, antibodies bind to specific surface antigen on leukocyte. After treatment with 1x RBC Lysis/Fixation Working solution, the erythrocytes are lysed under mild hypotonic conditions, keeping intact viability of white blood cells.