Chromatin Immunoprecipitation (ChIP) Assay Kit, BioAssay,

For immunoprecipitation of transcriptionally active chromatin from mammalian cells. Detection of gene or promoter of interest interest in immunoprecipitated chromatin must be empirically determined by the researcher. Quantitative PCR or Southern slot-blot analysis, using promotor-specific primer or probe, is recommended, Quantity: 22 Chromatin Immunoprecipitation (ChIP) assays. Kit Components: C5069-95A: Salmon Sperm DNA/Protein A Agarose, 1x1.5ml, C5069-95B: ChIP Dilution Buffer, 2x24ml , C5069-95C: Low Salt Immune Complex Wash Buffer, 1x24ml , C5069-95D: High Salt Immune Complex Wash Buffer, 1x24ml , C5069-95E: LiCl Immune Complex Wash Buffer, 1x24ml , C5069-95F: TE Buffer, 2x24ml , C5069-95G: EDTA, 1x250ul , C5069-95H: NaCl, 1x500ul , C5069-95J: Tris-HCl, 1x500ul , C5069-95K: SDS Lysis Buffer, 1x10ml, Storage and Stability: May be stored at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.