Buccutite(TM) Rapid PE-Texas Red Tandem Antibody Labeling Kit *Microscale Optimized for Labeling 25

Ex: 565 nm. Em: 600 nm. Soluble in: DMSO. PE-Texas Red is a popular color used in flow cytometry. Its primary absorption peak is at 565 nm with emission peak at 600 nm. AAT Bioquest offers this BuccutiteT rapid labeling kit to facilitate the PE-Texas Red tandem conjugations to antibodies and other proteins such as streptavidin and other secondary reagents. BuccutiteT PE-Texas Red Conjugation Kit provides a robust and convenient method to conjugate your antibodies with PE-Texas Red tandem. The kit includes a preactivated PE-Texas Red tandem and reaction buffer. The entire process only requires two simple mixings without further purification required. The conjugated antibody can be used in WB, ELISA and IHC applications. This kit is sufficient for 2 labeling reactions, each up to 25 ug of antibody. Considering the large size of PE (240 kDa), the amount of antibody used in a labeling reaction must always be less than the amount of RPE. The best ratio for any new antibody reagent must be determined by experimentation but 25 ug of IgG antibody for every 50 ug of RPE usually gives optimal results. Our kit provides preactivated PE-Texas Red to facilitate the PE-Texas Red tandem conjugations to antibodies and other proteins such as streptavidin and other secondary reagents. Our preactivated PE-Texas Red tandem is ready to conjugate, giving much higher yield than the conventionally tedious SMCC-based conjugation chemistry. In addition, our preactivated PE-Texas Red tandem is conjugated to a protein via its amino group that is abundant in proteins while SMCC chemistry targets the thiol group that has to be regenerated by the reduction of antibodies.