Buccutite(TM) Rapid APC-Cy5.5 Tandem Antibody Labeling Kit *Microscale Optimized for Labeling 100 ug

Ex: 651 nm. Em: 700 nm. Soluble in: DMSO. APC-Cy5.5 is a popular color used in flow cytometry. Its primary absorption peak is at 651 nm with emission peak at~700 nm. AAT Bioquest offers this BuccutiteT rapid labeling kit to facilitate the APC-Cy5.5 tandem conjugations to antibodies and other proteins such as streptavidin and other secondary reagents. BuccutiteT APC-Cy5.5 Tandem Conjugation Kit provides a robust and convenient method to conjugate antibodies with APC. The kit includes a preactivated APC-Cy5.5 tandem and reaction buffer. The conjugated antibody can be used in WB, ELISA and IHC applications. This kit is sufficient for 2 labeling reactions, each up to 100 ug of antibody. The best ratio for any new antibody reagent must be determined by experimentation. Our kit provides preactivated APC-Cy5.5 tandem to facilitate the APC-Cy5.5 tandem conjugations to antibodies and other proteins such as streptavidin and other secondary reagents. Our preactivated APC-Cy5.5 tandem is ready to conjugate, giving much higher yield than the conventionally tedious SMCC-based conjugation chemistry. In addition, our preactivated APC-Cy5.5 tandem is conjugated to a protein via its amino group that is abundant in proteins while SMCC chemistry targets the thiol group that has to be regenerated by the reduction of antibodies.