NZY First-Strand cDNA Synthesis Kit, separate oligos

NZY First-Strand cDNA Synthesis Kit, separate oligos, is a system that includes all the necessary components to synthesize first-strand cDNA, except the template RNA. Random hexamers and Oligo(dT)18 primers are provided in separate tubes to offer the convenience to choose the appropriate primer to initiate your reverse-transcription reaction. The resulting single-stranded cDNA is suitable for use in real-time quantitative Reverse Transcription PCR (RT-qPCR). NZY First-Strand cDNA Synthesis Kit, separate oligos, is formulated to provide high yields of full-length cDNA products and to increase sensitivity in RT-qPCR. Starting material can range from 10 pg up to 5 µg of total RNA. Besides random hexamers and Oligo(dT)18 primers, the kit includes NZYRT 2x Master Mix, no oligos, which contains dNTPs, MgCl2 and an optimized RT buffer, 10x Annealing Buffer and NZYRT Enzyme Mix. NZYRT Enzyme Mix includes both NZY Reverse Transcriptase (RNase H minus) and NZY Ribonuclease Inhibitor in order to protect RNA against degradation due to ribonuclease contamination. RNase H (from E. coli) is provided in a separate tube to specifically degrade the RNA G604template in cDNA-RNA hybrids after the first-strand cDNA synthesis. This procedure will improve the sensitivity of subsequent RT-qPCR reactions since PCR primers will bind more easily to the cDNA. Components: * NZYRT Enzyme Mix * NZYRT 2x Master Mix, no oligos * 10x Annealing Buffer * Random hexamer mix (50 ng/µL) * Oligo(dT)18 primer mix (50 µM) * NZY RNase H (E. coli) * DEPC-treated H2O. Shipping Conditions: Dry Ice. Storage Conditions: -85 °C to -15 °C. Application: cDNA synthesis, RT-PCR, RT-qPCR. Features: Flexible primer selection, High yield, Thermostable. Format: Kit, Liquid. Product Category: cDNA Synthesis. Reaction Temperature: 50 °C. Protocol Time: Approx. 60 min