NZY First-Strand cDNA Synthesis Kit

NZY First-Strand cDNA Synthesis Kit is a system that includes all the necessary components to synthesize first-strand cDNA, except the template RNA.The resulting single-stranded cDNA is suitable for use in real-time quantitative Reverse Transcription PCR (RT-qPCR). NZY First-Strand cDNA Synthesis Kit is formulated to provide high yields of full-length cDNA products and to increase sensitivity in RT-qPCR. Starting material can range from 10 pg up to 5 µg of total RNA. The kit includes a combination of random hexamers and oligo(dT)18 primers in order to increase sensitivity. The primers are included in the NZYRT 2x Master Mix, which also contains dNTPs, MgCl2 and an optimized RT buffer. NZYRT Enzyme Mix includes both the NZY Reverse Transcriptase (RNase H minus) and the NZY Ribonuclease Inhibitor in order to protect RNA against degradation due to ribonuclease contamination. RNase H (from E. coli) is provided in a separate tube to specifically degrade the RNA template in cDNA:RNA hybrids after the first-strand cDNA synthesis. This procedure will improve the sensitivity of subsequent RT-qPCR reaction since PCR primers will bind more easily to the cDNA. Components: * NZYRT Enzyme Mix * NZYRT 2x Master Mix * NZY RNase H (E. coli) * DEPC-treated H2O. Shipping Conditions: Dry Ice. Storage Conditions: -85 °C to -15 °C. Application: cDNA synthesis, RT-PCR, RT-qPCR. Features: Dual-primer: oligo(dT) + random hexamers, Fast, High yield, Thermostable. Format: Kit, Liquid. Product Category: cDNA Synthesis. Reaction Temperature: 50 °C. Protocol Time: Approx. 60 min