NZY First-Strand cDNA Synthesis Flexible Pack

NZY First-Strand cDNA Synthesis Flexible Pack is an optimized pack that includes the following components: the modified recombinant form of the Moloney Murine Leukemia Virus (MMuLV) Reverse Transcriptase (RNase H minus), which synthesizes the complementary DNA strand using either RNA or single-stranded DNA as template in a wide range of temperatures (37-50 °C), an optimized Reverse Transcriptases buffer, the NZY Ribonuclease Inhibitor for RNA protection against degradation due to ribonuclease contamination, a dNTPs mix, random hexamers and oligo(dT)18 primers, and finally, an E. coli RNase H to specifically degrade the RNA template in cDNA-RNA hybrids after the first-strand cDNA synthesis. This flexible pack offers the possibility to choose the primer to initiate the reaction: you can choose either the primers provided in separate tubes, or a gene-specific primer. The pack is sufficient for up to 200 first-strand cDNA synthesis reactions and it is optimized for RT reactions over a wide range of total RNA concentrations from 10 pg to 5 µg. Downstream applications include real-time PCR, standard PCR and microarrays. Components: * NZY Reverse Transcriptase (200 U/µL) * 10x Reaction buffer for Reverse Transcriptases * NZY Ribonuclease Inhibitor (40 U/µL) * dNTP Mix (10 mM each) * Random hexamer mix (50 ng/µL) * Oligo(dT)18 primer mix (50 µM) * NZY RNase H (E. coli) (5 U/µL) * DEPC-treated H2O. Shipping Conditions: Dry Ice. Storage Conditions: -85 °C to -15 °C. Application: cDNA synthesis, RT-PCR, RT-qPCR. Features: Fast, Flexible primer selection, High-yield, Thermostable. Format: Kit, Liquid. Product Category: cDNA Synthesis. Reaction Temperature: 50 °C. Protocol Time: Approx. 60 min