Human CD14+ Monocyte Isolation Kit

The Human CD14+ Monocyte Isolation Kit delivers a high purity population of classical human monocytes that are ready for downstream use. The BACSTM Microbubble workflow is exceptionally gentle on cells, helping to maintain the health and physiology of monocytes during enrichment., , Mechanism of Action: The Human CD14+ Monocyte Isolation Kit uses a negative selection method to remove unwanted cell populations and effectively enrich CD14+ monocytes. Unwanted cells are labeled with an optimized cocktail of biotinylated antibodies targeting CD3, CD5, CD15, CD16, CD19, CD20, CD56, CD57, CD66b, CD123, CD235ab, CD314., , How it works: The biotinylated antibody cocktail is added to the sample to label unwanted cells. BACSTM Microbubbles are added to the sample and bind to the antibody-labeled cells. Microbubbles bound to the undesired cells, quickly and gently float the undesired cells to the surface. CD14+ monocytes, the cells of interest, are pelleted through centrifugation. Microbubble-bound cells and supernatant are easily aspirated away, leaving untouched and enriched CD14+ monocytes healthy and ready for downstream use., , . Cell Type: Human CD14+ Monocytes, , . Sample Species and Source: Human MNCs, , . Cell Separation Method: Negative selection, , . Capacity: 1 billion MNCs, , Kit Includes:, , . BACSTM Streptavidin Microbubbles, , . Human CD14+ Monocyte Biotin Antibody Cocktail, , . Separation Buffer (Ca2+ and Mg2+ free PBS containing 2 mM EDTA, 0.5% biotin-free BSA), , . 5 ml Tubes