Zona pellucida sperm-binding protein 3 (ZP3), partial, human, recombinant

Organism: Homo sapiens (Human). Source: Mammalian cell. Expression Region: 23-387aa. Protein Length: partial. Tag Info: N-terminal 6xHis-Myc-tagged. Target Protein Sequence: QPLWLLQGGA SHPETSVQPV LVECQEATLM VMVSKDLFGT GKLIRAADLT LGPEACEPLV SMDTEDVVRF EVGLHECGNS MQVTDDALVY STFLLHDPRP VGNLSIVRTN RAEIPIECRY PRQGNVSSQA ILPTWLPFRT TVFSEEKLTF SLRLMEENWN AEKRSPTFHL GDAAHLQAEI HTGSHVPLRL FVDHCVATPT PDQNASPYHT IVDFHGCLVD GLTDASSAFK VPRPGPDTLQ FTVDVFHFAN DSRNMIYITC HLKVTLAEQD PDELNKACSF SKPSNSWFPV EGSADICQCC NKGDCGTPSH SRRQPHVMSQ WSRSASRNRR HVTEEADVTV GPLIFLDRRG DHEVEQWALP SDTSV. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP3 is essential for sperm binding and zona matrix formation. Reference: "Cloning and characterization of the human sperm receptor ligand ZP3: evidence for a second polymorphic allele with a different frequency in the Caucasian and Japanese populations."van Duin M., Polman J.E., Verkoelen C.C., Bunschoten H., Meyerink J.H., Olijve W., Aitken R.J.Genomics 14:1064-1070(1992). Function: The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP3 is essential for sperm binding and zona matrix formation.