Zona pellucida sperm-binding protein 3 (Zp3), mouse, recombinant

Organism: Mus musculus (Mouse). Source: E.coli. Expression Region: 23-351aa. Protein Length: Full Length of Mature Protein. Tag Info: C-terminal 6xHis-tagged. Target Protein Sequence: QTLWLLPGGT PTPVGSSSPV KVECLEAELV VTVSRDLFGT GKLVQPGDLT LGSEGCQPRV SVDTDVVRFN AQLHECSSRV QMTKDALVYS TFLLHDPRPV SGLSILRTNR VEVPIECRYP RQGNVSSHPI QPTWVPFRAT VSSEEKLAFS LRLMEENWNT EKSAPTFHLG EVAHLQAEVQ TGSHLPLQLF VDHCVATPSP LPDPNSSPYH FIVDFHGCLV DGLSESFSAF QVPRPRPETL QFTVDVFHFA NSSRNTLYIT CHLKVAPANQ IPDKLNKACS FNKTSQSWLP VEGDADICDC CSHGNCSNSS SSQFQIHGPR QWSKLVSRN. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Component of the zona pellucida, an extracellular matrix surrounding oocytes which mediates sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy. The zona pellucida is composed of 3 to 4 glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP3 is essential for sperm binding and zona matrix formation. Reference: "Crystal structure of the ZP-N domain of ZP3 reveals the core fold of animal egg coats." Monne M., Han L., Schwend T., Burendahl S., Jovine L. Nature 456:653-657(2008). Function: nan