Zinc transporter 8 (Slc30a8), mouse, recombinant

Organism: Mus musculus (Mouse). Source: in vitro E.coli expression system. Expression Region: 1-367aa. Protein Length: Full Length. Tag Info: N-terminal 10xHis-tagged. Target Protein Sequence: MEFLERTYLV NDQATKMYAF PLDRELRQKP VNKDQCPGDR PEHPEAGGIY HCHNSAKATG NRSSKQAHAK WRLCAASAIC FIFMVAEVVG GHVAGSLAIL TDAAHLLIDL TSFLLSLFSL WLSSRPPSKR LTFGWYRAEI LGALLSVLCI WVVTGVLLYL ACERLLYPDY QIQAGIMITV SGCAVAANIV LTMILHQRNF GYNHKDVQAN ASVRAAFVHA LGDVFQSISV LISALIIYFK PDYKIADPVC TFIFSILVLA STVMILKDFS ILLMEGVPKG LSYNSVKEII LAVDGVISVH SLHIWSLTVN QVILSVHVAT AASQDSQSVR TGIAQALSSF DLHSLTIQIE SAADQDPSCL LCEDPQD. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Facilitates the accumulation of zinc from the cytoplasm into intracellular vesicles, being a zinc-efflux transporter. May be a major component for providing zinc to insulin maturation and/or storage processes in insulin-secreting pancreatic beta-cells (By similarity). Reference: "Insulin crystallization depends on zinc transporter ZnT8 expression, but is not required for normal glucose homeostasis in mice." Lemaire K., Ravier M.A., Schraenen A., Creemers J.W., Van de Plas R., Granvik M., Van Lommel L., Waelkens E., Chimienti F., Rutter G.A., Gilon P., in't Veld P.A., Schuit F.C. Proc. Natl. Acad. Sci. U.S.A. 106:14872-14877(2009). Function: nan